PIN/LC8 is associated with cytosolic but not membrane-bound nNOS in the nitrergic varicosities of mice gut: implications for nitrergic neurotransmission
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منابع مشابه
PIN/LC8 is associated with cytosolic but not membrane-bound nNOS in the nitrergic varicosities of mice gut: implications for nitrergic neurotransmission.
This investigation demonstrates the presence and binding of the protein LC8 (described as "protein inhibitor of nNOS" or PIN in some reports) to different components of neuronal nitric oxide synthase (nNOS) in nitrergic varicosities of mice gut. Whole varicosity extracts showed three (320-, 250-, and 155-kDa) nNOS bands with anti-nNOS(1422-1433) antibody and a 10-kDa band with anti-LC8 antibody...
متن کاملActive and inactive pools of nNOS in the nerve terminals in mouse gut: implications for nitrergic neurotransmission.
Nitric oxide (NO) is responsible for nitrergic neurotransmission in the gut, and its release is dependent on its de novo synthesis by neuronal nitric oxide synthase (nNOS). The magnitude of NO synthesis and release during neurotransmission may be related to the fraction of catalytically active nNOS out of a larger pool of inactive nNOS in the nerve terminals. The purpose of the present study wa...
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Postsynaptic density (PSD) proteins in excitatory synapses are relatively immobile components, while there is a structured organization of mobile scaffolding proteins lying beneath the PSDs. For example, shank proteins are located further away from the membrane in the cytosolic faces of the PSDs, facing the actin cytoskeleton. The rationale of this organization may be related to important roles...
متن کاملMyosin Va plays a key role in nitrergic neurotransmission by transporting nNOSα to enteric varicosity membrane.
Nitrergic neurotransmission at the smooth muscle neuromuscular junctions requires nitric oxide (NO) release that is dependent on the transport and docking of neuronal NO synthase (nNOS) α to the membrane of nerve terminals. However, the mechanism of translocation of nNOSα in actin-rich varicosities is unknown. We report here that the processive motor protein myosin Va is necessary for nitrergic...
متن کاملRole of PSD 95 in membrane association and catalytic activity of nNOS α in nitrergic 1 varicosities in mice gut 2 3
23 We have recently shown that membrane association of nNOSα is critical in the regulation 24 of synthesis of nitric oxide (NO) during nitrergic neurotransmission. The purpose of this 25 study was to examine the role of the synapse associated proteins in membrane association 26 of nNOSα. Varicosities (swellings on terminal axons) were isolated from mice 27 gastrointestinal tract and examined fo...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Gastrointestinal and Liver Physiology
سال: 2008
ISSN: 0193-1857,1522-1547
DOI: 10.1152/ajpgi.90280.2008